Part:BBa_K3978000:Design

Yeast two-hybrid "prey" vector for expressing proteins fused to the GAL4 activation domain.

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1990
  Illegal PstI site found at 8
  Illegal PstI site found at 2036
  Illegal PstI site found at 4506
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1990
  Illegal NheI site found at 4496
  Illegal PstI site found at 8
  Illegal PstI site found at 2036
  Illegal PstI site found at 4506
  Illegal NotI site found at 4517
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1990
  Illegal BglII site found at 1898
  Illegal BamHI site found at 2018
  Illegal XhoI site found at 2030
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1990
  Illegal PstI site found at 8
  Illegal PstI site found at 2036
  Illegal PstI site found at 4506
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1990
  Illegal PstI site found at 8
  Illegal PstI site found at 2036
  Illegal PstI site found at 4506
  Illegal NgoMIV site found at 210
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 860
  Illegal BsaI.rc site found at 5714
  Illegal SapI site found at 4631
  

Design

The restriction sites EcoRI and BamHI were used to insert the gene. Saccharomyces cerevisiae codon optimization was used.

Source

Our sequence comes from SnapGene.